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Updated: Jul 5, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Quadruplex PCR for simultaneous detection of serotype, biotype, toxigenic potential, and central regulating factor of
Hemant Kumar Khuntia1, Bibhuti Bhusan Pal, Guru Prasada Chhotray
1Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar, Orissa, India.
Abstract:
A quadruplex PCR was developed for the simultaneous detection of genes specific for Vibrio cholerae O1 and/or O139 serogroup (wbe and/or wbf), cholera toxin A subunit (ctxA), toxin-coregulated pilus (tcpA), and central regulating protein ToxR (toxR) in a single tube reaction. This is a simple, rapid, and accurate approach for the detection of toxigenic V. cholerae O1 and/or O139 and can prevent the rapid spread of the disease by early detection.
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