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Updated: Jul 5, 2026

Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures
Published on: March 28, 2018
Ephrin-A5 promotes the formation of terminal thalamocortical arbors
Daniela Uziel1, Sven Mühlfriedel, Jürgen Bolz
1University of Jena, Institute for General Zoology, Erberstrasse 1, Jena, Germany.
Abstract:
Ephrins-A5 are expressed in the cortical target layer of thalamic afferents at the time when these axons form terminal arbors. Previous in-vitro studies provided evidence that ephrin-A5 supports the branching of thalamic axons, but there is no direct in-vivo evidence for such a growth-promoting effect. Here we examined thalamocortical projections in ephrins-A5 deficient mice. Our results demonstrate that the laminar specificity of thalamic afferents in ephrin-A5 mutants remains preserved, but axonal arbor formation is greatly reduced. Thus, ephrin-A5 specifically regulates branch formation of thalamic axons, but does not affect target layer selection. Ephrin-A5-mutant mice are, therefore, a unique model to study the effects of reduced thalamic innervation on the assembly of cortical circuits and sensory processing.
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