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An Efficient Method for Adenovirus Production
Published on: June 10, 2021
Gene delivery by adenoviruses.
Renny T Franceschi1, Chunxi Ge
1Department of Periodontics & Oral Medicine, School of Dentistry, and Biological Chemistry, School of Medicine, University of Michigan, Ann Arbor, MI, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 9, 2008
Summary
Adenoviruses are effective gene delivery vectors for cell transduction and gene overexpression. This chapter details adenovirus construction and in vivo delivery for measuring osteogenic activity.
Area of Science:
- Biotechnology
- Molecular Biology
- Gene Therapy
Background:
- Adenoviruses offer advantages as gene delivery vectors, including high transduction efficiency in various cell types.
- Their ease of construction and purification into high-titer stocks makes them suitable for research applications.
Purpose of the Study:
- To describe procedures for adenovirus construction.
- To outline methods for adenovirus delivery to in vivo sites.
- To detail the measurement of osteogenic activity using adenovirus vectors.
Main Methods:
- Adenovirus vector construction protocols.
- In vivo administration techniques for viral delivery.
- Assays for quantifying osteogenic activity.
Main Results:
- High-titer adenovirus stocks can be generated efficiently.
- Adenovirus vectors facilitate gene delivery to target tissues in vivo.
- Osteogenic activity can be reliably measured following adenovirus-mediated gene delivery.
Conclusions:
- Adenoviruses are versatile tools for gene delivery and expression studies.
- The described methods enable robust evaluation of gene function in vivo.
- This approach is valuable for preclinical research in bone biology.
