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Updated: Jul 5, 2026

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy
Published on: September 6, 2024
In situ nuclear organization of regulatory machinery
Shirwin M Pockwinse1, Sayyed K Zaidi, Ricardo F Medina
1Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA.
Abstract:
Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
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