Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Bioluminescent Bacterial Imaging In Vivo
05:06

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Bioluminescent imaging in bone.

Yoram Zilberman1, Yossi Gafni, Gadi Pelled

  • 1Skeletal Biotechnology Laboratory, Hebrew University-Hadassah Medical Center, Jerusalem, Israel.

Methods in Molecular Biology (Clifton, N.J.)
|May 9, 2008
PubMed
Summary

Bioluminescent imaging allows for quantitative monitoring of gene expression in bone development and repair, both in vitro and in vivo. This technique utilizes transgenic mice with luciferase reporter genes for effective bone gene therapy analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A theranostic 3D ultrasound imaging system for high resolution image-guided therapy.

Theranostics·2022
Same author

Encapsulation of Human-Bone-Marrow-Derived Mesenchymal Stem Cells in Small Alginate Beads Using One-Step Emulsification by Internal Gelation: In Vitro, and In Vivo Evaluation in Degenerate Intervertebral Disc Model.

Pharmaceutics·2022
Same author

Collagenase Administration into Periodontal Ligament Reduces the Forces Required for Tooth Extraction in an Ex situ Porcine Jaw Model.

Journal of functional biomaterials·2022
Same author

Applications of Ultrasound-Mediated Gene Delivery in Regenerative Medicine.

Bioengineering (Basel, Switzerland)·2022
Same author

PTH-Induced Bone Regeneration and Vascular Modulation Are Both Dependent on Endothelial Signaling.

Cells·2022
Same author

Intradiscal quantitative chemical exchange saturation transfer MRI signal correlates with discogenic pain in human patients.

Scientific reports·2021

Area of Science:

  • Molecular biology
  • Biomedical imaging
  • Regenerative medicine

Background:

  • Accurate monitoring of gene expression is vital for understanding osteogenesis and advancing bone gene therapy.
  • Traditional methods like histology and micro-PET/CT have limitations in quantitative gene expression analysis in bone.
  • Bioluminescent imaging (BLI) offers a powerful solution for in vitro and in vivo gene expression tracking.

Purpose of the Study:

  • To provide a comprehensive overview of protocols for detecting bioluminescence in bone development and repair.
  • To highlight the advantages of BLI for quantitative gene expression monitoring in osteogenesis research.
  • To detail the use of luciferase reporter genes in transgenic mouse models for bone studies.

Main Methods:

  • Utilizing transgenic mice harboring the luciferase reporter gene under an osteogenesis-specific promoter.

More Related Videos

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging
08:04

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Published on: April 14, 2011

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice
18:40

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Published on: October 16, 2014

Related Experiment Videos

Last Updated: Jul 5, 2026

Bioluminescent Bacterial Imaging In Vivo
05:06

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging
08:04

Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging

Published on: April 14, 2011

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice
18:40

Combined In vivo Optical and µCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Published on: October 16, 2014

  • Implementing bioluminescent imaging techniques for quantitative detection of light emission.
  • Describing protocols for analyzing bioluminescence signals in bone tissue both in vitro and in vivo.

    • Main Results:

    • Bioluminescent imaging enables efficient and quantitative monitoring of gene expression in bone.
    • The described protocols facilitate the study of bone development and repair processes.
    • Transgenic mouse models with luciferase reporters are effective for in vivo gene expression analysis.

    Conclusions:

    • Bioluminescent imaging is the premier system for quantitative gene expression monitoring in bone development and repair.
    • This approach is essential for advancing bone gene therapy research and preclinical studies.
    • Detailed protocols are crucial for the successful application of BLI in bone regeneration research.