[Clinical trial of periodontal tissue regeneration]

A goal of periodontal therapy is to regenerate periodontal tissue, which had been destroyed by periodontal diseases. Transplantation of bone marrow-derived mesenchymal stem cells (MSCs) into periodontal defects would be a useful option for periodontal tissue regeneration. We have developed a novel method for periodontal therapy using MSCs. Human bone marrow cells are obtained from the iliac crest and MSCs are isolated and expanded in vitro at Cell and Tissue Engineering Center in Hiroshima University Hospital. MSCs are mixed with 2% Atelocollagen at final concentration of 2 x10(7) cells/mL. These MSCs in Atelocollagen are transplanted into periodontal osseous defects at the periodontal surgery.