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Updated: Jul 5, 2026
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Overexpressing and Purifying a Toxic Nuclease from Escherichia coli
Published on: August 29, 2025
[The expression, purification and activity analysis of the rice chitinase gene in Escherichia coli]
Aikui Chen1, Lujun Yu, Jianming Fan
1Biology Department, Guangdong Institute of Education, Guangzhou 510303, China.
Abstract:
The cloned cDNA sequence of rice (Oryza sativa L. Cpslo17) chitinase gene Oschi was cloned, (which was registered in GenBank, the accession number: EU045451) ligated with the expression vector pGEX-4T-1, and transformed into E. coli BL21(DE3). The expression of Oschi was induced by IPTG, and the conditions were optimized. After purification the in vitro activity of Oschi chitinase was analyzed, and the results indicated that it could efficiently degrade chitin.
