Related Experiment Video
Updated: Jul 5, 2026
Establishment of a Surgically-induced Model in Mice to Investigate the Protective Role of Progranulin in Osteoarthritis
Published on: February 25, 2014
Influence of mouse genetic background on wear particle-induced in vivo inflammatory osteolysis
1Department of Orthopaedics, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, People's Republic of China.
Objective And Design:
Genetics may influence wear particle-induced inflammatory osteolysis after joint replacement. In the present work, mice with three different genetic backgrounds were used to test this hypothesis.
Treatment:
C57BL/6J, Balb/c and Kunming mouse were used. Each kind of mouse was divided into those receiving 30 mg UHMWPE particle implantation onto the calvariae and those receiving a sham operation.
Methods:
Mice of each group were sacrificed one week after surgery. Calvariae were harvested for immunological assay of TNF-alpha and IL-1 beta secretion in supernatants of calvariae organ culture and histological analysis of calvarial sagittal suture osteolysis and osteoclastogenesis.
Results:
Although UHMWPE particles induced obvious calvarial sagittal suture osteolysis and osteoclastogenesis in all strains as compared with their corresponding control mice, the most significant change was found in C57BL/6J mice, less severe in Balb/c mice and much less severe in Kunming mice. In agreement with pathological findings, UHMWPE particles induced the highest IL-1 beta secretion in C57BL/6J mice, compared with Balb/c and Kunming mice. However, no difference was observed concerning TNF-alpha secretion among these mice.
Conclusion:
Our data suggests that genetics had a significant influence on wear particle-induced inflammation, osteoclastogenesis and osteolysis. The influence of genetic background on implant life in patients with joint replacement warrants further investigation.
Insights
Genetic background significantly impacts wear particle-induced osteolysis. C57BL/6J mice showed the most severe bone loss and inflammation, highlighting genetics
Area of Science:
- Biomedical Engineering
- Immunology
- Orthopedic Research
Background:
- Inflammatory osteolysis, a complication of joint replacement, may be influenced by host genetics.
- Understanding genetic predispositions to osteolysis is crucial for improving implant longevity.
Purpose of the Study:
- To investigate the hypothesis that genetic background influences wear particle-induced inflammatory osteolysis.
- To compare the inflammatory and osteolytic responses to ultra-high-molecular-weight polyethylene (UHMWPE) particles across different mouse strains.
Main Methods:
- Three mouse strains (C57BL/6J, Balb/c, Kunming) were subjected to UHMWPE particle implantation on calvariae or sham surgery.
- Calvariae were analyzed for tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) secretion.
- Histological analysis assessed calvarial sagittal suture osteolysis and osteoclastogenesis.
Main Results:
- UHMWPE particles induced osteolysis and osteoclastogenesis in all strains compared to controls.
- C57BL/6J mice exhibited the most severe osteolysis and highest IL-1 beta secretion.
- Balb/c and Kunming mice showed progressively less severe responses; TNF-alpha secretion did not differ significantly.
Conclusions:
- Host genetics significantly influence wear particle-induced inflammation, osteoclastogenesis, and osteolysis.
- These findings suggest genetic background may affect joint replacement implant survival.
- Further research is warranted to explore the clinical implications of genetic factors in joint replacement outcomes.
