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Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model
Published on: June 20, 2025
Gene expression in human peripheral blood mononuclear cells upon acute ischemic stroke
C Grond-Ginsbach1, M Hummel, T Wiest
1Dept. of Neurology, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany. Caspar_Grond-Ginsbach@med.uni-heidelberg.de
Journal of Neurology
|May 10, 2008
Summary
Acute ischemic stroke (AIS) patients show altered gene expression in circulating immune cells compared to healthy individuals. While no significant differences were found between AIS patients and stroke survivors, specific inflammatory genes and PDE4D were notably different in AIS patients.
Area of Science:
- Immunology
- Genomics
- Neuroscience
Background:
- Ischemic stroke triggers a systemic inflammatory response.
- Characterizing this response at the gene expression level is crucial for understanding stroke pathophysiology.
Purpose of the Study:
- To investigate gene expression profiles in circulating mononuclear leukocytes of acute ischemic stroke (AIS) patients.
- To compare gene expression between AIS patients, stroke survivors, acute traumatic brain injury (ATBI) patients, and healthy controls.
Main Methods:
- RNA analysis of peripheral blood mononuclear cells (PBMCs) from AIS patients (n=20) and controls using Affymetrix GeneChips.
- Comparison of gene expression signatures between AIS patients, stroke survivors (n=15), ATBI patients (n=15), and healthy subjects (n=15).
Main Results:
- No significant single gene expression differences were found between AIS patients and stroke survivors or ATBI patients.
- AIS patients showed significantly different expression of phosphodiesterase 4D (PDE4D) compared to healthy subjects (p=0.012).
- Differential gene expression was observed in inflammatory response gene groups (GO:0006954) between AIS patients and healthy subjects, notably involving FPRL1, IL1RN, and C3AR1.
Conclusions:
- Transcriptome analysis revealed no significant changes in circulating mononuclear cells between AIS patients and stroke survivors 24 hours post-stroke.
- Measurable differences in PDE4D and a set of inflammatory response genes were identified when comparing AIS patients to healthy controls.
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