Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Antiprotozoal Agents

Antiprotozoal Agents

Leishmaniasis is a widespread parasitic disease caused by several Leishmania species. It affects millions of people each year and remains a major public health problem in endemic regions. First-line treatment relies on pentavalent antimonials, including meglumine antimoniate and sodium stibogluconate. Even so, how these drugs work has not been fully clear, especially their interaction with parasite-specific biochemical pathways. One key target is trypanothione reductase (TR), an enzyme that...

Pharmacovigilance

Pharmacovigilance

Post-marketing surveillance is a critical component of pharmaceutical regulation, often uncovering unanticipated adverse drug reactions (ADRs) once a drug is widely used over an extended period.
This process, termed pharmacovigilance, aims to detect, evaluate, and minimize harmful effects related to medication use. The data collection for pharmacovigilance depends on spontaneous reporting systems, where healthcare professionals or patients voluntarily report suspected ADRs.
In some cases, there...

Drug Regulation

Drug Regulation

Drug regulation encompasses the management of drug usage by evaluating its safety and efficacy through assessments conducted by regulatory authorities. Regrettably, the history of drug regulation is marred by several catastrophic events. One such incident is the Elixir Sulfanilamide tragedy, in which the toxic compound diethyl glycol was included in a sweet-tasting medication, leading to numerous fatalities. This event prompted the enactment of the Food, Drug, and Cosmetic Act in 1938. Under...

Inhibitors Of Virion Release

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...

Antifungal Agents

Antifungal Agents

Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...

Pharmaceutical Equivalents

Pharmaceutical Equivalents

As defined by regulatory standards, pharmaceutical equivalents require generic drug products to have identical dosage forms and chemically identical active pharmaceutical ingredients (APIs). They must adhere to compendial or applicable standards for potency, content uniformity, disintegration times, and dissolution rates. In the case of modified-release dosage forms, variations in drug content are permissible as long as the delivered amount remains consistent with the innovator drug product.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Activation loop sequences confer substrate specificity to phosphoinositide 3-kinase alpha (PI3Kalpha ). Functions of lipid kinase-deficient PI3Kalpha in signaling.

The Journal of biological chemistry·2001

Same author

Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors.

Molecular and cellular biology·2000

Same author

Identification of germline missense mutations and rare allelic variants in the ATM gene in early-onset breast cancer.

Genes, chromosomes & cancer·1999

Same author

Proteomic definition of normal human luminal and myoepithelial breast cells purified from reduction mammoplasties.

Proceedings of the National Academy of Sciences of the United States of America·1999

Same author

Human phosphoinositide 3-kinase C2beta, the role of calcium and the C2 domain in enzyme activity.

The Journal of biological chemistry·1998

Same author

1H NMR structure of an antifungal gamma-thionin protein SIalpha1: similarity to scorpion toxins.

Proteins·1998

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

Flavopiridol Hoechst AG.

¹Ludwig Institute for Cancer Research, Courtauld Building, 91 Riding House Street, London, W1P 8BT, UK. marketa@ludwig.ucl.ac.uk

Idrugs : the Investigational Drugs Journal

|May 10, 2008

Summary

This summary is machine-generated.

Flavopiridol, a synthetic flavonoid, inhibits cell division and induces apoptosis in cancer cells. Clinical trials are underway for lymphoma and multiple myeloma, showing promising results in preclinical studies.

More Related Videos

Determination of 45 Pesticides in Avocado Varieties by the QuEChERS Method and Gas Chromatography-Tandem Mass Spectrometry

Determination of 45 Pesticides in Avocado Varieties by the QuEChERS Method and Gas Chromatography-Tandem Mass Spectrometry

Published on: December 8, 2023

Flavonoid Content During the Growth and Floral Development of Calendula officinalis L.

Flavonoid Content During the Growth and Floral Development of Calendula officinalis L.

Published on: June 27, 2025

Related Experiment Videos

Last Updated: Jul 5, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

Published on: August 14, 2019

Determination of 45 Pesticides in Avocado Varieties by the QuEChERS Method and Gas Chromatography-Tandem Mass Spectrometry

Determination of 45 Pesticides in Avocado Varieties by the QuEChERS Method and Gas Chromatography-Tandem Mass Spectrometry

Published on: December 8, 2023

Flavonoid Content During the Growth and Floral Development of Calendula officinalis L.

Flavonoid Content During the Growth and Floral Development of Calendula officinalis L.

Published on: June 27, 2025

Area of Science:

Oncology
Pharmacology
Molecular Biology

Background:

Flavopiridol is a synthetic flavonoid developed for cancer treatment.
It functions as a cyclin-dependent kinase (CDK) inhibitor, arresting cell division and inducing apoptosis.

Purpose of the Study:

To evaluate the potential of flavopiridol in cancer therapy.
To assess its efficacy and safety in preclinical and clinical settings.

Main Methods:

Phase II clinical trial for lymphoma and multiple myeloma.
Ex vivo experiments on chronic lymphoblastic leukemia cells.
In vitro pharmacokinetic studies and CDK inhibition assays.
Preclinical toxicology studies in rats and dogs.

Main Results:

Flavopiridol demonstrated dose-dependent CDK2 inhibition and apoptosis in leukemia cells.
Hepatic biotransformation to glucuronide via UDP-glucuronosyltransferases observed.
IC50 for CDK inhibition is 0.4 mM.
Preclinical studies showed dose-related leukopenia and organ toxicity, with gastrointestinal and bone marrow toxicity being dose-limiting.

Conclusions:

Flavopiridol exhibits anti-cancer properties by inhibiting cell division and inducing apoptosis.
Ongoing clinical trials will further define its therapeutic potential.
Toxicity profile requires careful management in clinical application.