Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Production of Pharmaceuticals

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

Bioequivalence studies: Biowaivers

Bioequivalence studies: Biowaivers

In certain scenarios, in vitro dissolution tests can replace in vivo bioequivalence studies. This is particularly true when a drug product, though available in varying strengths, maintains proportional similarity in its active and inactive ingredients. In such cases, the need for in vivo bioequivalence studies for lower strength variants may be waived, provided dissolution tests and in vivo studies on the highest strength yield satisfactory results.Bioequivalence can be indicated through...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lifetime measurements of the neutron-rich N = 30 isotones 50Ca and 51Sc: orbital dependence of effective charges in the fp shell.

Physical review letters·2009

Same author

Apamin increases 5-HT cell firing in raphe dorsalis and extracellular 5-HT levels in amygdala: a concomitant in vivo study in anesthetized rats.

Brain research·2009

Same author

Giant dipole resonance in the hot and thermalized 132Ce nucleus: damping of collective modes at finite temperature.

Physical review letters·2006

Same author

Forced swimming test and fluoxetine treatment: in vivo evidence that peripheral 5-HT in rat platelet-rich plasma mirrors cerebral extracellular 5-HT levels, whilst 5-HT in isolated platelets mirrors neuronal 5-HT changes.

Experimental brain research·2002

Same author

Can voltammetry measure nitrogen monoxide (NO) and/or nitrites?

Journal of neuroscience methods·2001

Same author

Electrochemical evidence that lacidipine stimulates release of nitrogen monoxide in rat aorta.

Neuroscience letters·2001

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

SB-204269 SmithKline Beecham.

¹Glaxo Wellcome SpA, Pharmacology Department, Via Fleming 4, 37100 Verona, Italy. FC20377@ggr.co.uk

Idrugs : the Investigational Drugs Journal

|May 10, 2008

Summary

SB-204269 is a novel anticonvulsant agent in development for epilepsy. Its unique mechanism of action does not involve ATP-sensitive potassium channels, distinguishing it from related compounds.

Area of Science:

Neuroscience
Pharmacology

Background:

SB-204269 is an anticonvulsant agent developed by SmithKline Beecham.
It is structurally related to cromakalim, a benzopyran ATP-sensitive potassium channel (KATP) opener, but possesses opposite stereochemistry.

Purpose of the Study:

To investigate the anticonvulsant properties and mechanism of action of SB-204269.
To determine if SB-204269's activity involves ATP-sensitive potassium channels.

Main Methods:

Pre-clinical studies evaluating anticonvulsant activity.
Binding assays to assess affinity for ATP-sensitive potassium channels.
Stereochemical analysis and comparison with enantiomer SB-204268.

Main Results:

SB-204269 demonstrated anticonvulsant activity.

More Related Videos

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

Related Experiment Videos

Last Updated: Jul 5, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

No significant affinity for the ATP-sensitive potassium channel binding site was observed for SB-204269.

The opposite enantiomer, SB-204268, showed no anticonvulsant activity.

Conclusions:

SB-204269 represents a mechanistically novel anticonvulsant agent.
The mechanism of action of SB-204269 is not believed to involve ATP-sensitive potassium channels.