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Characterization of the Pseudomonas aeruginosa transposable phage D3112 [corrected] left-end regulatory region
C Autexier1, S Wragg-Légaré, M S DuBow
1Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.
Biochimica Et Biophysica Acta
|January 17, 1991
Abstract:
The nucleotide sequence (2682 bp) of the left end of the Mu-like transposable bacteriophage D3112 cts15 from Pseudomonas aeruginosa was determined. A 720 bp open reading frame (ORF) is located on the bottom strand (positions 892-173), potentially encoding a polypeptide of 240 residues (Mr = 26,329). Specific binding of Escherichia coli Integration Host Factor (IHF) to a site located 907-922 bp from the D3112 left end suggests the existence of a P. aeruginosa IHF and its role, as in Mu, in the regulation of phage development.