Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Disclosure is not destigmatization: rethinking AI and mental health stigma.

Frontiers in public health·2026

Same author

Evaluation of prognostic factors and outcomes in primary versus secondary myeloid sarcoma.

Human pathology·2026

Same author

Application of Hi-C sequencing to detect oncogene rearrangements for diagnosis and treatment of large B-cell lymphoma.

Blood advances·2026

Same author

Cytogenetic and Molecular Analysis of a "Double-Hit" RUNX1 Including a RUNX1 p.Trp279* and a Cryptic Novel t(6;21)(q25;q22)/RUNX1::ARID1B in Acute Myeloid Leukemia.

Genes, chromosomes & cancer·2026

Same author

Genetic Profile, Treatment Response, and Outcomes of BCR::ABL1-Positive Mixed-Phenotype Acute Leukemia: A Study From the BCR::ABL1 Pathology Group.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2026

Same author

Research progress and clinical application of functional magnetic resonance imaging in otolaryngology-head and neck diseases.

Frontiers in neurology·2026

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

Immunophenotypic study of basophils by multiparameter flow cytometry.

Xiaohong Han¹, Jeffrey L Jorgensen, Archana Brahmandam

¹Department of Hematopathology, The University of Texas M. D. Anderson Cancer Center, Houston, USA.

Archives of Pathology & Laboratory Medicine

|May 10, 2008

Summary

This study defines normal basophil immunophenotypes and identifies aberrations in chronic myelogenous leukemia (CML). Flow cytometry effectively distinguishes CML basophils from myeloblasts, aiding diagnosis.

More Related Videos

Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

Related Experiment Videos

Last Updated: Jul 5, 2026

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

Area of Science:

Hematology
Immunology
Flow Cytometry

Background:

The immunophenotypic profile of basophils is not fully established.
Immunophenotypic changes in chronic myelogenous leukemia (CML) require further characterization.

Purpose of the Study:

To define the comprehensive immunophenotypic spectrum of normal basophils.
To assess basophil immunophenotypic aberrations in CML patients.

Main Methods:

Utilized 4-color flow cytometry.
Compared peripheral blood or bone marrow basophils from 20 healthy controls and 15 CML patients.

Main Results:

Established normal basophil markers (e.g., CD9, CD13, CD123 bright) and negative markers (e.g., CD19, CD34).
Identified 1-5 immunophenotypic aberrations in CML basophils, most commonly CD38 underexpression, CD64 aberrant expression, and CD123 underexpression.
CD34 and CD117 expression indicated basophilic precursors; myeloblasts showed distinct profiles.

Conclusions:

Flow cytometry can identify basophil immunophenotypic aberrations in CML.
Flow cytometry effectively differentiates basophils from myeloblasts in CML cases.