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Updated: Jul 5, 2026

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
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Probing pineal-specific gene expression with transgenic zebrafish.

Daisuke Kojima1, John E Dowling, Yoshitaka Fukada

  • 1Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.

Photochemistry and Photobiology
|May 10, 2008
PubMed
Summary
This summary is machine-generated.

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We developed a transgenic zebrafish model to study the pineal gland

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • The zebrafish pineal gland is crucial for the neuroendocrine system and contains photoreceptors.
  • The pineal expression-promoting element (PIPE) regulates pineal-specific gene expression.
  • Understanding PIPE's regulatory mechanisms requires in vivo genetic tools.

Purpose of the Study:

  • To generate a transgenic zebrafish line for studying PIPE-dependent gene expression in the pineal gland.
  • To visualize PIPE-mediated gene regulation in vivo.
  • To establish a tool for identifying genetic factors affecting pineal gene expression.

Main Methods:

  • Creation of a transgenic zebrafish line (Tg(P(20)-rh/P:gfp)) expressing GFP under the control of a promoter with 20 PIPE repeats.

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  • Observation of GFP fluorescence in the pineal gland and retina.
  • Knock-down of the otx5 transcription factor using antisense morpholino.
  • Pilot screen for mutations affecting pineal GFP expression.

    • Main Results:

    • The Tg(P(20)-rh/P:gfp) zebrafish line shows detectable and reproducible GFP fluorescence in the larval pineal gland by 5 days postfertilization.
    • GFP expression is dependent on PIPE in the pineal gland but independent in retinal rod photoreceptors.
    • Knock-down of otx5 suppressed pineal GFP expression.
    • Mutations attenuating pineal GFP were identified.

    Conclusions:

    • The Tg(P(20)-rh/P:gfp) zebrafish line effectively visualizes PIPE-dependent gene expression in the pineal gland.
    • This transgenic model is a valuable tool for investigating the genetic regulation of the zebrafish pineal gland.
    • The line can be used to detect deficits in pineal gene expression.