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Related Experiment Video

Updated: Jul 5, 2026

Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues
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Investigating CXCR4 expression in canine appendicular osteosarcoma.

T M Fan1, A M Barger, R L Fredrickson

  • 1Department of Veterinary Clinical Medicine, University of Illinois at Urbana-Champaign, Urbana, IL 61802-4714, USA. t-fan@uiuc.edu

Journal of Veterinary Internal Medicine
|May 10, 2008
PubMed
Summary

Canine osteosarcoma (OSA) cells express CXCR4, a chemokine receptor involved in cell migration. This receptor is present in most primary canine OSA tumors, suggesting its role in cancer progression and metastasis.

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Area of Science:

  • Veterinary Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Chemokine receptors (CXCRs) regulate leukocyte homing and cellular processes like directional migration.
  • CXCRs on cancer cells influence tissue-specific metastasis.
  • Canine and pediatric osteosarcoma (OSA) share biological similarities, including metastasis patterns.

Purpose of the Study:

  • To investigate CXCR4 expression in canine OSA cell lines and naturally occurring tumors.
  • To determine if CXCR4 signaling contributes to directional cell migration in canine OSA.

Main Methods:

  • Assessed CXCR4 expression using RT-PCR, Western blot, flow cytometry, and immunocytochemistry in vitro.
  • Investigated CXCR4-mediated directional migration with a commercial assay in vitro.

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  • Evaluated in vivo CXCR4 expression in primary tumors and pulmonary metastases using immunohistochemistry.

    • Main Results:

    • Canine OSA cell lines express CXCR4 mRNA and protein in vitro.
    • CXCL12-induced CXCR4 ligation promoted directional migration of canine OSA cells.
    • CXCR4 protein was expressed in most primary canine OSA tumors (8/11) but fewer pulmonary metastases (2/8).

    Conclusions:

    • Canine OSA cells express CXCR4, and its signaling pathway is implicated in directional cell migration.
    • CXCR4 is present in the majority of primary canine osteosarcoma tumors, indicating its potential role in the disease.