Related Experiment Video
Updated: Jul 5, 2026
Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue-Resident Macrophages In Vivo in Mice
Published on: February 16, 2024
[Construction and targeting study of eukaryotic expression vector modulated by a macrophage-specific promoter]
Yang-an Wen1, Jin-ying Guo, Xiao-lin Yu
1Faculty of Laboratory Medicine, Chongqing Medical University, the Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing 400016, China. wenyangan0105@yahoo.com.cn
Aim:
To construct a macrophage-specific eukaryotic expression vector and to investigate its expressing specificity.
Methods:
Macrophage-specific promoter was synthesized using PCR, and substituted the CMV promoter of eukaryotic expression vector pEGFP-N1 to construct a recombinant vector (pSP-GFP), which were cotransfected with pERFP-N1 vector into different cell lines. The green fluorescent protein (GFP) and red fluorescent protein (RFP) was observed by fluorescence microscopy, and the target specificity of macrophage-specific promoter was judged by comparison of expressed level of GFP and RFP in various cell lines.
Results:
The macrophage-specific eukaryotic expression vector was successfully constructed, which showed strong activity and specificity only in macrophage cells.
Conclusion:
The constructed pSP-GFP vector was macrophage-specific, which are potential to targeted gene therapy of intracellular bacterial infection.
