Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high affinity and are together...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Updates in the Diagnosis and Management of Hypertension: A Review of Recent Guidelines.

British journal of hospital medicine (London, England : 2005)·2026
Same author

The neuropeptide neuromedin U receptor NMUR-1 buffers insulin receptor signaling in bacteria-dependent C. elegans survival.

PLoS genetics·2026
Same author

In vivo characterization of a patient CACNA1A variant reveals paradoxical synaptic effects.

bioRxiv : the preprint server for biology·2026
Same author

Activity induced GEM-4/Copine expression inhibits gap junctions and promotes thermosensory plasticity.

bioRxiv : the preprint server for biology·2026
Same author

AFD thermosensory neurons mediate tactile-dependent locomotion modulation in <i>C. elegans</i>.

eLife·2026
Same author

Transsynaptic linking of calcium channels and postsynaptic receptors at a dyadic synapse.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Jul 5, 2026

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors
07:51

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors

Published on: November 14, 2014

An RNAi screen identifies genes that regulate GABA synapses.

Amy B Vashlishan1, Jon M Madison, Mike Dybbs

  • 1Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.

Neuron
|May 10, 2008
PubMed
Summary
This summary is machine-generated.

Researchers screened for genes affecting muscle activity to find those regulating GABA transmission. This identified 90 genes, including 21 linked to seizure disorders, offering potential epilepsy candidates.

More Related Videos

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
09:40

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Published on: February 13, 2012

Related Experiment Videos

Last Updated: Jul 5, 2026

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors
07:51

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors

Published on: November 14, 2014

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
09:40

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

Published on: February 13, 2012

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • GABAergic synapses are crucial for neural circuit development and function.
  • Dysregulation of GABA transmission is linked to neurological conditions like epilepsy.

Purpose of the Study:

  • To identify novel genes regulating GABAergic transmission using a C. elegans model.
  • To investigate the role of these genes in muscle activity and potential links to epilepsy.

Main Methods:

  • Conducted an RNA interference (RNAi) screen in C. elegans to identify genes affecting muscle activity.
  • Utilized electrophysiological recordings and synaptic imaging to analyze GABAergic and cholinergic transmission.
  • Investigated humoral pathways and protein kinases involved in GABAergic modulation.

Main Results:

  • Identified 90 genes that modulate C. elegans muscle activity upon inactivation.
  • 21 of the identified genes have prior associations with seizure syndromes.
  • Several genes were found to selectively regulate GABA transmission without significantly affecting excitatory transmission.

Conclusions:

  • The screen effectively identified candidate genes for epilepsy and related disorders.
  • Discovered conserved signaling pathways, including humoral pathways and protein kinases, that regulate GABA transmission.
  • These findings highlight potential therapeutic targets for epilepsy and other cognitive/psychiatric disorders.