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Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product
D P Bottaro1, J S Rubin, D L Faletto
1Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Science (New York, N.Y.)
|February 15, 1991
Abstract:
Hepatocyte growth factor (HGF) is a plasminogen-like protein thought to be a humoral mediator of liver regeneration. A 145-kilodalton tyrosyl phosphoprotein observed in rapid response to HGF treatment of intact target cells was identified by immunoblot analysis as the beta subunit of the c-met proto-oncogene product, a membrane-spanning tyrosine kinase. Covalent cross-linking of 125I-labeled ligand to cellular proteins of appropriate size that were recognized by antibodies to c-met directly established the c-met product as the cell-surface receptor for HGF.