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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
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Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
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Anti-CD25 antibodies affect cytokine synthesis pattern of human dendritic cells and decrease their ability to prime

K Mnasria1, C Lagaraine, F Velge-Roussel

  • 1JE 2448, Cellules Dendritiques et Greffe, Faculté de Medicine, Université François Rabelais, Equipe 10 Bd Tonnelle, 37032 Tours Cedex, France.

Journal of Leukocyte Biology
|May 10, 2008
PubMed
Summary

Anti-CD25 antibodies impact dendritic cells (DC) by altering cytokine production and T helper cell priming, potentially explaining their effectiveness in preventing transplant rejection. This research reveals new effects on DC function and the role of IL-2 signaling.

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Area of Science:

  • Immunology
  • Transplantation Immunology

Background:

  • Anti-CD25 monoclonal antibodies are crucial for preventing acute allograft rejection in clinical transplantation.
  • The effects of these antibodies on T lymphocytes are well-documented, but their impact on human dendritic cells (DC) remains largely unexplored.
  • The specific role of Interleukin-2 (IL-2) in DC functions is not fully understood.

Purpose of the Study:

  • To investigate the effects of anti-CD25 monoclonal antibodies on human monocyte-derived dendritic cells (moDC).
  • To elucidate the role of IL-2 signaling in DC activation and function.
  • To understand how anti-CD25 antibodies influence DC-mediated alloimmune responses.

Main Methods:

  • Human moDC were stimulated with lipopolysaccharide (LPS).
  • Expression of CD25 and IL-2 receptor (IL-2R) beta and gamma chains on LPS-matured DC was analyzed.
  • The impact of anti-CD25 antibodies on DC cytokine production (IL-12, IL-1, TNF-alpha, IL-6, IFN-gamma, IL-10) was assessed.
  • T helper cell priming ability and alloimmune polarization by DC were evaluated.
  • Expression of co-stimulatory molecules (CD86, CD80, CD83, HLADR, CD40) was analyzed.

Main Results:

  • LPS stimulation strongly induced CD25 expression and IL-2R components on moDC.
  • Anti-CD25 antibodies significantly decreased IL-12, IL-1, TNF-alpha, IL-6, and IFN-gamma production while increasing IL-10 synthesis by DC.
  • These cytokine profile changes diminished DC's T helper priming capacity and shifted alloimmune responses towards T helper 2 (TH2) polarization.
  • Crucially, anti-CD25 antibodies did not impede the upregulation of key DC activation markers (CD86, CD80, CD83, HLADR, CD40) induced by LPS.

Conclusions:

  • This study reveals previously unrecognized effects of anti-CD25 monoclonal antibodies on human dendritic cells.
  • These findings suggest that anti-CD25 antibodies modulate DC function, potentially contributing to their clinical efficacy in transplantation.
  • The research also provides insights into the function of IL-2 signaling in human DC activation.