Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Copy Number Variation: A Substrate for Plant Adaptation and Stress Response in Arabidopsis.

G3 (Bethesda, Md.)·2026

Same author

Muller's ratchet and gene duplication.

Theoretical population biology·2025

Same author

Detecting adaptive changes in gene copy number distribution accompanying the human out-of-Africa expansion.

Human genome variation·2024

Same author

Copy number variation and population-specific immune genes in the model vertebrate zebrafish.

eLife·2024

Same author

Polarization-resolved mode evolution in TMI-limited Yb-doped fiber amplifiers using a novel high-speed Stokes polarimeter.

Optics express·2024

Same author

Mitigation of transverse mode instability by modal birefringence in polarization-maintaining fibers.

Optics express·2023

Same journal

Regulatory Effects of Cooperativity and Signal Profile on Adaptive Dynamics in Incoherent Feedforward Loop Networks.

In silico biology·2025

Same journal

scAN1.0: A reproducible and standardized pipeline for processing 10X single cell RNAseq data.

In silico biology·2023

Same journal

Modelling speciation: Problems and implications.

In silico biology·2022

Same journal

Where Do CABs Exist? Verification of a specific region containing concave Actin Bundles (CABs) in a 3-Dimensional confocal image.

In silico biology·2022

Same journal

Network analysis of host-pathogen protein interactions in microbe induced cardiovascular diseases.

In silico biology·2022

Same journal

Multiscale modeling of tumor response to vascular endothelial growth factor (VEGF) inhibitor.

In silico biology·2022

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Self-alignments to detect mutually exclusive exon usage.

Martin Stephan¹, Friedrich Möller, Thomas Wiehe

¹Institut für Molekularbiologie und Bioinformatik, Charité Berlin, Germany. martin.stephan@charite.de

In Silico Biology

|May 10, 2008

Summary

This summary is machine-generated.

Researchers identified a new method to detect mutually exclusive exon usage (MEEU) in genes. This approach aids in finding alternatively spliced genes, crucial for understanding genome complexity.

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Related Experiment Videos

Last Updated: Jul 5, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

The human genome has fewer genes than expected, but produces many more transcripts.
Alternative splicing significantly increases transcript diversity, with over 60% of human genes undergoing this process.
Mutually exclusive exon usage (MEEU) is a specific type of alternative splicing.

Purpose of the Study:

To develop a novel method for detecting genes with mutually exclusive exon usage.
To identify new gene candidates exhibiting MEEU, particularly in D. melanogaster.
To address limitations of classical gene-finding programs in identifying MEEU.

Main Methods:

Development of a gene-finding method based on local exon similarity.
Screening of the entire D. melanogaster genome to identify MEEU candidates.
Comparison with existing gene-finding tools to highlight the new method's advantages.

Main Results:

Identification of five new D. melanogaster genes with MEEU.
Confirmation of eight previously described MEEU cases in D. melanogaster.
Discovery of 1703 additional candidate regions for mutually exclusive exons.

Conclusions:

The new method effectively detects genes with mutually exclusive exon usage.
This research expands the known repertoire of alternatively spliced genes.
The findings contribute to a deeper understanding of genome complexity and gene regulation.