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Correlation between CLSI, EUCAST and Etest methodologies for amphotericin B and fluconazole antifungal susceptibility

A L R Claudino1, R F Peixoto, M S C Melhem

  • 1Departamento de Ciências Biológicas, Universidade Federal de Alfena (UNIFAL-MG), Alfenas-MG, Brazil.

Die Pharmazie
|May 13, 2008
PubMed

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Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...