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Updated: Jul 5, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Highly sensitive detection of pharmaceutical compounds in biological fluids using capillary electrophoresis coupled
Julie Schappler1, Aline Staub, Jean-Luc Veuthey
1Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences - EPGL, University of Geneva, University of Lausanne, Bd d'Yvoy 20, 1211 Geneva 4, Switzerland.
Abstract:
Due to its selectivity and sensitivity, capillary electrophoresis coupled to laser-induced fluorescence has evolved as a useful analytical tool for determining drugs in biological samples. This paper describes a simple, sensitive, efficient, and rapid method for analyzing propranolol without derivatization in plasma by CE-LIF using a diode solid-state laser at 266nm. An experimental design methodology was assessed for the investigation of electrophoretic parameters and a Box-Behnken design was selected to optimize both sensitivity and efficiency. The CE-LIF method was linear over the concentration range of 50-850ngmL(-1) and successfully applied to a real forensic sample.
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