Analysis of cultivated potato (Solanum tuberosum) using intermicrosatellite amplification

Twelve potato (Solanum tuberosum) cultivars were analysed using intermicrosatellite amplification with two 5′-anchored repeat primers. Amplification products were resolved on polyacrylamide gels. In total, 24 polymorphic bands were scored and used to construct a dendrogram showing the relationships between the cultivars. The three late 19th - early 20th century cultivars studied were grouped together, as were the three accessions directly descended from S. tuberosum cv. Maris Piper. These results suggest that intermicrosatellite amplification is a potentially useful tool for both phenetic and genotyping studies. Key words : potato, microsatellites, intermicrosatellite amplification, genotyping.