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Inheritance of random amplified polymorphic DNA markers in cassava (Manihot esculenta Crantz)
Genome
|October 1, 1996
Summary
Randomly amplified polymorphic DNA (RAPD) markers are useful for genetic mapping in crops. This study identified numerous single-dose markers for linkage analysis in Manihot.
Area of Science:
- Genetics
- Molecular Biology
- Plant Breeding
Background:
- Developing molecular genetic maps is crucial for crop improvement.
- Randomly amplified polymorphic DNA (RAPD) markers offer a method for genetic analysis.
- Manihot (cassava) has limited classical and molecular genetic studies.
Purpose of the Study:
- To investigate the informativeness and inheritance of RAPD markers.
- To assess RAPD utility for parent comparison in developing a molecular genetic map.
- To identify markers for linkage analysis in Manihot.
Main Methods:
- Analysis of RAPD markers in an intraspecific F1 progeny from two heterozygous parents.
- Screening of primers for polymorphic products and single-dose (SD) markers.
- Construction of linkage groups based on SD RAPD marker segregation.
Main Results:
- RAPD markers proved useful for comparing parental lines and developing a genetic map.
- 328 single-dose markers segregating 1:1 were identified from 722 primers.
- A significant proportion of monomorphic markers segregated in a 3:1 ratio, consistent with dominant markers.
Conclusions:
- RAPD markers are valuable tools for genetic mapping and linkage analysis in Manihot.
- The study provides numerous markers for future genetic studies in this crop.
- This research facilitates the development of molecular genetic maps for Manihot breeding programs.
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