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Related Experiment Video

Updated: Jul 5, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
10:28

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Haploid regeneration from tetraploid wheat using maize pollen.

A Sarrafi, N Amrani, G Alibert

    Genome
    |February 1, 1994
    PubMed
    Summary

    Maize pollen successfully produced haploid plants from tetraploid wheat. This method, using 2,4-D treatment, demonstrates a new potential for generating haploid wheat (Triticum turgidum) for genetic studies and breeding.

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    Trihybrid Crosses02:27

    Trihybrid Crosses

    Trihybrid Crosses
    Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
    The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...

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    Area of Science:

    • Plant breeding
    • Genetics
    • Crop science

    Background:

    • Haploid plants are crucial for genetic research and breeding in wheat.
    • Producing haploid plants from tetraploid wheat (Triticum turgidum) can be challenging.
    • Utilizing interspecific crosses offers potential for novel haploid production methods.

    Purpose of the Study:

    • To investigate the efficacy of using maize (Zea mays) pollen for haploid induction in tetraploid wheat.
    • To evaluate the role of 2,4-D in enhancing haploid embryo survival.
    • To assess the genetic control of key traits in tetraploid wheat x maize crosses.

    Main Methods:

    • Crosses were performed between 21 tetraploid wheat genotypes and a maize F1 hybrid.
    • Embryos were rescued from pollinated wheat spikes, with 2,4-D applied to enhance survival.
    • Haploid plants were identified, and chromosome doubling was attempted.

    Main Results:

    • Embryo recovery rates ranged from 2.34-14.14 per 100 developed ovaries.
    • Sixty-nine haploid plants were obtained, with 56 successfully chromosome-doubled.
    • General combining ability was significant, indicating additive genetic control for ovary development and haploid production.

    Conclusions:

    • Maize pollen can be effectively used to produce haploid plants from diverse tetraploid wheat genotypes.
    • The application of 2,4-D significantly aids in embryo survival and haploid plant regeneration.
    • This interspecific hybridization approach presents a viable strategy for haploid induction in tetraploid wheat.

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