[Study of immunolipo-sodium morrhuate applied on human hemangioma endothelial cells in vitro]
Zhuang-Qun Yang1, Yong Song, Jun-Bo Tu
1Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Xi'an Jiao Tong University, Xi'an, Shanxi Province, China. yzhqun@mail.xjtu.edu.cn
Purpose:
To explore the method of preparing immunolipo-sodium morrhuate and evaluate its effect on human hemangioma endothelial cells in vitro.
Methods:
Using SPDP((N-Succinimidyl-3-(2-pyridyldithio)) propionate) as cross-linker, anti-VEGFR2/KDR monoclone antibody was combined to the liposome surface to prepare immunolipo-sodium morrhuate by extruding method, and then its effect on human hemangioma endothelial cells in vitro was observed by laser scanning confocal microscope, inverted microscope, Gimsa staining, transmission electron microscope, MTT and flow cytometry.
Results:
The average diameter of the immunoliposome was 122.9 nm, which had a very good stability when compared with normal liposome, it had stronger and faster combining ability, its potential to induce apoptosis was much more prominent, and its toxic effect on human hemangioma endothelial cells was gentle, which was similar to normal liposome.
Conclusion:
We have prepared immunolipo-sodium morrhuate successfully, which has very good specific initiative targetting ability in vitro and can induce pervasive apoptosis of human hemangioma endothelial cells.
