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Updated: Jul 5, 2026

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
Published on: December 17, 2015
[The role of Porphyromonas gingivalis invasion on E-selectin expression in endothelial cells]
Dong-Mei Zhang1, Ya-Ping Pan, Li Lin
1Department of Periodontology, School of Stomatology, China Medical University, Shenyang, Liaoning Province, China.
Purpose:
To evaluate the role of Porphyromonas gingivalis(P.gingivalis)ATCC 33277 and W83 invasion on the mRNA and protein expression of E-selectin in vascular endothelial cells.
Methods:
A cell culture model of human umbilical vein endothelial cells was used in vitro. E-selectin mRNA was measured by reverse transcription-polymerase chain reaction; the protein expression of E-selectin was detected by Western blot assay. The data was analyzed with SAS 8.12 software package for repeated measures ANOVA.
Results:
P.gingivalis not only enhanced mRNA expression of E-selectin, but also enhanced the protein level of E-selectin.Compared to the control, the relative mRNA expression of E-selectin after P.gingivalis ATCC 33277/W83 invasion at 4-hour, 8-hour, 24-hour or exposed to TNFalpha was 294.0%/326.4%(P<0.05),179.3%/224.6%(P<0.05),126.7%/128.3% and 365.5%(P<0.05), respectively. The relative protein expression of E-selectin was 295.4%/343.6% (P<0.05), 386.3%/394.3%(P<0.05), 238.7%/296.4% (P<0.05) and 413.8% (P<0.05), respectively. The effect of P.gingivalis virulent strain W83 was more strongly than that of avirulent strain ACTT 33277.
Conclusion:
P.gingivalis plays an important role in the pathogenesis and progression of atherosclerosis partly through upregulation of E-selectin.
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