Influence of lithium on second messenger accumulation in NG108-15 cells

Treatment of NG108-15 cells with bradykinin for 10 min resulted in a reduction of phosphatidylinositol levels with no change in the other inositol lipids. Accompanying this was a small increase in diacylglycerol but no modification in the level of inositol phosphates. Addition of bradykinin to cells incubated for 30 min in buffer containing lithium did not cause a further decrease in the labeling of phosphatidylinositol, but was accompanied by an increase in mass of the three inositol phosphates. The increase was about 150% for inositol monophosphate, 40% for inositol bisphosphate and 80% for inositol trisphosphate. Under these conditions there was a marked increase in the ability of bradykinin to elevate the content of diacylglycerol. Moreover lithium by itself induced an increase in diacylglycerol six times as great as the increase in total inositol phosphates. The excess diacylglycerol may have its origin in phospholipids other than phosphoinositides.