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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Spatial and temporal influences on bacterial profiling of forensic soil samples
Melissa S Meyers1, David R Foran
1Forensic Science Program, School of Criminal Justice, Michigan State University, East Lansing, MI 48824, USA.
Journal of Forensic Sciences
|May 13, 2008
Summary
Forensic soil analysis using bacterial DNA profiling shows promise. However, temporal variations in bacterial communities mean that time must be considered for accurate sample differentiation.
Area of Science:
- Microbiology
- Forensic Science
- Environmental Science
Background:
- Bacterial communities in soil can potentially differentiate forensic soil samples.
- Effectiveness depends on habitat uniqueness, intra-habitat heterogeneity, and temporal changes.
Purpose of the Study:
- To assess the impact of habitat type and temporal variation on soil bacterial communities for forensic applications.
- To determine the reliability of bacterial profiling in forensic soil analysis.
Main Methods:
- Terminal Restriction Fragment Length Polymorphism (TRFLP) analysis was used.
- Soils from five diverse habitats were sampled over one year.
- Sampling included monthly central locations and quarterly peripheral locations.
Main Results:
- Greatest bacterial similarities were found within the same habitat's collection locations.
- Least similarity was observed among different habitats.
- Significant temporal variations and overlap in bacterial similarity indices occurred throughout the year.
Conclusions:
- Bacterial DNA profiling is a potentially valuable tool for forensic soil analysis.
- Temporal dynamics of soil bacterial communities are a critical factor to consider for accurate differentiation.
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