Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures
13:38

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures

Published on: April 11, 2017

Tissue-to-cellular level deformation coupling in cell micro-integrated elastomeric scaffolds.

John A Stella1, Jun Liao, Yi Hong

  • 1Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA.

Biomaterials
|May 13, 2008
PubMed
Summary

Engineered tissue scaffolds show that cellular deformation depends non-linearly on scaffold microstructural changes, not just applied strain. This finding is crucial for understanding tissue growth and remodeling in engineered tissues.

Related Concept Videos

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic value of cystatin C in acute kidney injury among patients with sepsis: a systematic review and meta-analysis.

Frontiers in medicine·2026
Same author

A Chemically Defined Four-Component Self-Adjuvanting Tn Vaccine Activating Mincle, FcγR, and CD206 for Enhanced Antitumor Immunity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Targeting TROP2 in drug-tolerant persister cells delays EGFR tyrosine kinase inhibitor resistance in non-small-cell lung cancer.

Cancer cell·2026
Same author

Rare earth nanoparticles: Illuminating the future of precision photodynamic therapy in oncology.

Journal of photochemistry and photobiology. B, Biology·2026
Same author

MRI-based habitat radiomics for preoperative prediction of axillary pathological complete response in breast cancer after neoadjuvant therapy: a multicenter study.

Frontiers in oncology·2026
Same author

Single-nucleus analysis reveals human-specific oligodendrocyte polarization and conserved neuronal responses after severe traumatic brain injury.

Nature communications·2026

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Cell Mechanics

Background:

  • Reproducing the extracellular matrix and cellular deformation coupling in native tissues is a key challenge in engineered tissues.
  • This meso-micro scale phenomenon significantly impacts tissue growth and remodeling.
  • Current methods struggle to replicate this complex interplay.

Purpose of the Study:

  • To investigate cellular deformations within a 3D elastomeric fibrous scaffold using a novel electrospinning and electrospraying technique.
  • To quantify the relationship between scaffold deformation, microarchitecture changes, and cellular deformation.
  • To understand the implications for tissue development and remodeling in engineered constructs.

Main Methods:

  • Fabrication of polymer fiber scaffolds with integrated vascular smooth muscle cells via electrospinning and electrospraying.

Related Experiment Videos

Last Updated: Jul 5, 2026

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures
13:38

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures

Published on: April 11, 2017

  • Application of controlled biaxial stretch to scaffold specimens.
  • Simultaneous quantification of 3D cellular deformations and local fiber microarchitecture using advanced imaging techniques.

    • Main Results:

    • Local fiber geometry exhibited affine behavior, predictable by macro-scaffold deformations.
    • Cellular deformations depended non-linearly on microarchitectural changes and ceased when scaffold fibers straightened at large strains.
    • Scaffold microstructural changes, driven by applied strain, were the dominant factor in cellular deformation.

    Conclusions:

    • Cellular deformation in engineered tissues is not solely dictated by applied scaffold strain.
    • Microstructural changes within the scaffold play a critical, non-linear role in modulating cellular responses.
    • These findings have fundamental implications for designing and interpreting experiments on de-novo tissue development and remodeling.