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Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jul 5, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Total internal reflection resonance light scattering at solid/liquid interfaces.

Yao-Ji Tang1, Ying Chen, Min-Na Yao

  • 1Department of Chemistry and The Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Siming South Road 422, Xiamen 361005, China.

Journal of Pharmaceutical and Biomedical Analysis
|May 13, 2008
PubMed
Summary
This summary is machine-generated.

Total internal reflection resonance light scattering (TIR-RLS) is a novel technique for studying solid/liquid interfaces. This method effectively analyzes interactions and differentiates macromolecular states at interfaces.

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Published on: January 24, 2018

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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
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Published on: May 3, 2022

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Total Internal Reflection Absorption Spectroscopy (TIRAS) for the Detection of Solvated Electrons at a Plasma-liquid Interface
08:50

Total Internal Reflection Absorption Spectroscopy (TIRAS) for the Detection of Solvated Electrons at a Plasma-liquid Interface

Published on: January 24, 2018

Area of Science:

  • Interface science
  • Biophysical chemistry
  • Spectroscopy

Background:

  • Total internal reflection (TIR) is an interface-specific optical phenomenon.
  • Resonance light scattering (RLS) offers high sensitivity for detecting analytes.
  • Studying macromolecular behavior at solid/liquid interfaces is crucial in various scientific fields.

Purpose of the Study:

  • To introduce and validate the combined Total Internal Reflection Resonance Light Scattering (TIR-RLS) technique for interfacial analysis.
  • To investigate the behavior of a mixture of TPPS and Bovine Serum Albumin (BSA) at the solid/liquid interface.
  • To assess the efficacy of TIR-RLS in studying interfacial interactions and macromolecular states.

Main Methods:

  • Development and application of a novel Total Internal Reflection Resonance Light Scattering (TIR-RLS) system.
  • Experimental analysis of the TPPS and BSA mixture at the solid/liquid interface.
  • Utilizing the interface-specific nature of TIR and the sensitivity of RLS.

Main Results:

  • The study successfully demonstrated the application of TIR-RLS at the solid/liquid interface.
  • Preliminary results show the technique's capability in observing the behaviors of TPPS and BSA mixtures.
  • The method proved effective in distinguishing different states of macromolecules at the interface.

Conclusions:

  • TIR-RLS is a promising new technique for interfacial studies.
  • The combined approach offers a sensitive and specific method for analyzing macromolecular interactions and states at solid/liquid interfaces.
  • This technique has potential applications in biophysics and materials science.