Rutin determination in pharmaceutical formulations using a carbon paste electrode modified with...

Area of Science:

• Electrochemistry
• Analytical Chemistry
• Materials Science

Background:

• Rutin, a flavonoid, possesses significant biological activities.
• Accurate quantification of rutin is crucial for pharmaceutical quality control.
• Development of sensitive and selective electrochemical sensors is an active research area.

Purpose of the Study:

• To develop a modified carbon paste electrode for the determination of rutin.
• To investigate the role of poly(vinylpyrrolidone) (PVP) in enhancing rutin adsorption.
• To evaluate the analytical performance of the developed sensor for rutin quantification.

Main Methods:

• Fabrication of a carbon paste electrode modified with poly(vinylpyrrolidone) (PVP).
• Electrochemical characterization using cyclic and linear sweep voltammetry.
• Optimization of experimental parameters including composition, accumulation time, scan rate, and supporting electrolyte (pH).
• Validation of the method using pharmaceutical samples.

Main Results:

• The modified electrode exhibited an oxidation peak for rutin at +0.87 V vs. Ag/AgCl.
• Optimal composition was 75:15:10% (w/w/w) graphite powder:Nujol:PVP.
• Linearity was observed for rutin concentrations from 3.9 x 10(-7) to 1.3 x 10(-5)M (r=0.9991).
• A low detection limit of 1.5 x 10(-7)M was achieved.
• High recovery rates (98.3-101.7%) and low relative standard deviation (3.3%) were obtained.
• Successful application in the determination of rutin in pharmaceutical formulations.

Conclusions:

• The PVP-modified carbon paste electrode is a sensitive and selective sensor for rutin determination.
• The developed method provides accurate and reliable results, comparable to standard methods.
• This sensor offers a promising tool for routine analysis of rutin in pharmaceutical products.