Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of hydrodynamic, mechanical, and interfacial stress during downstream processing on adeno-associated virus serotype 8 and 9.

Journal of pharmaceutical sciences·2025

Same author

Kinetic Modeling of the Antibody Disulfide Bond Reduction Reaction With Integrated Prediction of the Drug Load Profile for Cysteine-Conjugated ADCs.

Biotechnology and bioengineering·2024

Same author

Kinetic models towards an enhanced understanding of diverse ADC conjugation reactions.

Frontiers in bioengineering and biotechnology·2024

Same author

HIV ribonuclease H: continuing the search for small molecule antagonists.

HIV therapy·2024

Same author

Rational downstream development for adeno-associated virus full/empty capsid separation - A streamlined methodology based on high-throughput screening and mechanistic modeling.

Journal of chromatography. A·2024

Same author

From amaurotic idiocy to biochemically defined lipid storage diseases: the first identification of GM1-Gangliosidosis.

Free neuropathology·2023

Search research articles

Related Experiment Video

Updated: Feb 13, 2026

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

Hexosaminidase assays.

Michaela Wendeler¹, Konrad Sandhoff

¹LIMES, Membrane Biology and Lipid Biochemistry Unit, c/o Kekulé-Institut für Organische Chemie und Biochemie der Universität Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.

Glycoconjugate Journal

|May 14, 2008

Summary

Beta-hexosaminidases are crucial lysosomal enzymes. This review details convenient assay methods for diagnosing GM2 gangliosidoses, studying enzyme function, and screening for inhibitors.

Area of Science:

Biochemistry
Enzymology
Molecular Biology

Background:

Beta-hexosaminidases (EC 3.2.1.52) are lysosomal enzymes critical for glycoconjugate metabolism.
Deficiency in beta-hexosaminidases causes GM2 gangliosidoses, a group of lysosomal storage disorders.
Altered hexosaminidase levels are increasingly linked to inflammatory conditions.

Purpose of the Study:

To review convenient assay procedures for beta-hexosaminidases.
To cover applications including diagnostics, research, and inhibitor screening.
To provide an overview of established and novel assay techniques.

Main Methods:

Utilizing fluorogenic or chromogenic artificial substrates.
Employing the physiological glycolipid substrate GM2.

More Related Videos

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Published on: November 1, 2013

Related Experiment Videos

Last Updated: Feb 13, 2026

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Published on: November 1, 2013

Discussing alternative and high-throughput screening techniques.

Main Results:

Established assay systems are vital for diagnosing GM2 gangliosidoses and carrier screening.
In vitro assays are essential for characterizing knockout mice and enzyme variants.
Novel methods facilitate high-throughput screening for enzyme inhibitors.

Conclusions:

Convenient and reliable beta-hexosaminidase assays are indispensable for clinical diagnostics and fundamental research.
These assays support the investigation of lysosomal storage disorders and enzyme mechanisms.
Recent advancements enable efficient screening for potential therapeutic inhibitors.