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Related Concept Videos

Candidiasis01:20

Candidiasis

Candidiasis is a fungal infection caused by opportunistic species of Candida. It can affect various anatomical sites, including the skin, oral cavity, nails, and genitourinary tract. Among its forms, vaginal candidiasis is the most common type of mucosal infection. It typically results from the overgrowth of Candida albicans in the vaginal mucosa. Under normal conditions, C. albicans exists as a commensal organism within the vaginal microbiota, regulated by the dominance of lactobacilli, which...

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Candida dubliniensis and Candida albicans display surface variations consistent with observed intergeneric

M A Jabra-Rizk1, W A Falkler, W G Merz

  • 1Department of Oral Medicine and Department of Oral and Cranofacial Biological Sciences Dental School, University of Maryland, Baltimore, USA. mrizk@umaryland.edu.

Revista Iberoamericana De Micologia
|May 14, 2008
PubMed
Summary

Candida dubliniensis exhibits consistent cell surface fibrils, unlike Candida albicans, potentially enhancing its adherence and colonization in oral environments. This suggests C. dubliniensis may have a competitive advantage over C. albicans.

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Area of Science:

  • Microbiology
  • Cell Biology
  • Mycology

Background:

  • Yeast adherence to microorganisms and epithelial cells is crucial for colonization.
  • Comparative studies suggest surface differences between Candida dubliniensis and Candida albicans.
  • Oral bacteria coaggregation highlights potential variations in yeast surface properties.

Purpose of the Study:

  • To investigate morphologic variations in the cell surface of Candida dubliniensis and Candida albicans.
  • To test the hypothesis that cell surface structures differ between these two Candida species.
  • To correlate cell surface morphology with adherence properties.

Main Methods:

  • Transmission electron microscopy (TEM) was employed to visualize yeast cell surfaces.
  • Candida dubliniensis and Candida albicans were cultured at different temperatures (25°C, 37°C, 45°C).
  • Yeast cells were fixed using a combination of dimethylsulfoxide, alcian blue, paraformaldehyde, and glutaraldehyde.

Main Results:

  • Both species displayed a fibrillar outer layer on their cell walls.
  • Candida albicans fibrils varied in length and density with growth temperature, being longer and more packed at 37°C.
  • Candida dubliniensis fibrils remained short, blunt, and consistent across different temperatures, resembling C. albicans grown at 25°C.

Conclusions:

  • Candida dubliniensis possesses constant cell surface characteristics, likely contributing to hydrophobicity.
  • These stable surface properties may confer an ecological advantage for C. dubliniensis in oral environments.
  • C. dubliniensis may exhibit enhanced attachment and competition compared to C. albicans due to its consistent fibrillar layer.