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Related Experiment Video

Updated: Jul 5, 2026

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
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Multicenter evaluation of Neo-Sensitabs, a standardized diffusion method for yeast susceptibility testing.

A J Carrillo-Muñoz1, L Abarca, G Quindós

  • 1Dept. Microbiology, ACIA, Barcelona, Spain and Spanish Committee for Antifungal Testing Standardization (Asociación Española de Micología). alfonso@aciam.com.

Revista Iberoamericana De Micologia
|May 14, 2008
PubMed
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This summary is machine-generated.

The Neo-Sensitabs agar diffusion method offers a reliable and easy approach for antifungal susceptibility testing. This method demonstrates high reproducibility, making it suitable for evaluating drugs like fluconazole and itraconazole.

Area of Science:

  • Mycology
  • Clinical Microbiology
  • Pharmaceutical Sciences

Background:

  • Antifungal susceptibility testing is crucial for guiding treatment decisions.
  • Standardized and reliable methods are needed for in vitro antifungal testing.
  • Evaluating novel antifungal agents requires robust susceptibility assays.

Purpose of the Study:

  • To evaluate the Neo-Sensitabs agar diffusion method for antifungal susceptibility testing.
  • To assess the reproducibility of the Neo-Sensitabs method across multiple laboratories.
  • To compare the Neo-Sensitabs method with other commercialized antifungal susceptibility testing methods.

Main Methods:

  • The Neo-Sensitabs agar diffusion method was evaluated using 33 reference fungal strains.
  • Fourteen laboratories participated in the study, testing various antifungal agents including 5-fluorocytosine, amphotericin B, nystatin, fluconazole, itraconazole, ketoconazole, and tioconazole.

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  • Inhibition zone diameters were measured to classify strains as susceptible, intermediate, or resistant.

    • Main Results:

    • The Neo-Sensitabs method demonstrated high intra- and interlaboratory reproducibility (97.1%).
    • The method was found to be easy to perform and reliable for antifungal susceptibility testing.
    • Neo-Sensitabs showed superiority compared to other commercialized methods, particularly for triazole derivatives like fluconazole and itraconazole.

    Conclusions:

    • The Neo-Sensitabs agar diffusion method is a reliable and reproducible technique for in vitro antifungal susceptibility testing.
    • This method is particularly valuable for evaluating triazole antifungals such as fluconazole and itraconazole.
    • Neo-Sensitabs offers a practical and effective alternative for antifungal sensitivity testing in clinical laboratories.