Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Classification System

Microbial Classification System

Classification is the process of organizing organisms into hierarchically inclusive groups based on their phenotypic similarities or evolutionary relationships. A species comprises one or more strains, and closely related species are grouped into genera. Genera are further classified into families, families into orders, orders into classes, and so forth, up to the domain level, which is the broadest taxonomic rank derived from a combination of phenotypic and genotypic data.The nomenclature of...

Diversity of Protists III

Diversity of Protists III

Rhizaria are a diverse group of unicellular protists characterized by their threadlike cytoplasmic extensions known as pseudopodia. These structures aid in both locomotion and feeding, giving Rhizaria an amoeboid appearance. Their amoeboid morphology once led to taxonomic confusion, but molecular phylogenetics has clarified their evolutionary placement and emphasized their shared use of pseudopodia despite divergent lineages.This clade comprises diverse lineages such as Chlorarachniophyta,...

Methods of Classification and Identification

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...

Phylogenetic Species Concept in Microbiology

Phylogenetic Species Concept in Microbiology

The phylogenetic species concept (PSC) is a framework used to delineate species based on evolutionary relationships, emphasizing shared ancestry and diagnosable genetic traits. Unlike morphological or biological species concepts, the PSC is particularly advantageous for microbial taxonomy, where traditional reproductive or phenotypic criteria often fall short due to the prevalence of asexual reproduction, minimal morphological differentiation, and widespread horizontal gene transfer among...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Introduction to Plant Diversity

Introduction to Plant Diversity

From Water to Land

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Translated article] Clinical-Epidemiological Profile, and Treatment Response in Relation to Associated Atopic Comorbidity in Atopic Dermatitis. Experience From the BIOBADATOP Registry.

Actas dermo-sifiliograficas·2025

Same author

Clinical-Epidemiological Profile, and Treatment Response in Relation to Associated Atopic Comorbidity in Atopic Dermatitis. Experience From the BIOBADATOP Registry.

Actas dermo-sifiliograficas·2024

Same author

Consensus on the Diagnostic Algorithm for Chronic Nodular Prurigo.

Actas dermo-sifiliograficas·2024

Same author

[Translated article] BIOBADATOP Spanish Atopic Dermatitis Registry: Description and Early Findings.

Actas dermo-sifiliograficas·2023

Same author

BIOBADATOP Spanish Atopic Dermatitis Registry: Description and Early Findings.

Actas dermo-sifiliograficas·2023

Same author

Cuentos Lunares: Poems and Flash Fiction That Save Lives - A Euromelanoma Project During the COVID-19 Pandemic.

Actas dermo-sifiliograficas·2021

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Genotyping of Plant and Animal Samples without Prior DNA Purification

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

[Dermatophytes classification by PCR.].

M Pereiro Ferreirós¹, A Flórez Menéndez, J Toribio Pérez

¹Departamento de Dermatología, Complejo Hospitalario Universitario, Facultad de Medicina, Universidad de Santiago de Compostela, Spain. manuelpe@usc.es.

Revista Iberoamericana De Micologia

|May 14, 2008

Summary

This study introduces a faster molecular method for fungal taxonomy. Analyzing mitochondrial DNA (mtDNA) sequences helps differentiate fungal species more efficiently than previous long laboratory procedures.

More Related Videos

Field Identification of Matricaria chamomilla using a Portable qPCR System

Field Identification of Matricaria chamomilla using a Portable qPCR System

Published on: October 10, 2020

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Published on: September 28, 2021

Related Experiment Videos

Last Updated: Jul 5, 2026

Genotyping of Plant and Animal Samples without Prior DNA Purification

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

Field Identification of Matricaria chamomilla using a Portable qPCR System

Field Identification of Matricaria chamomilla using a Portable qPCR System

Published on: October 10, 2020

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Published on: September 28, 2021

Area of Science:

Mycology
Molecular Biology
Genetics

Context:

Fungal taxonomy relies heavily on molecular biology techniques.
Existing molecular methods for fungal identification are often lengthy laboratory procedures.
There is a need for more efficient molecular approaches in fungal taxonomy.

Purpose:

To develop and validate a rapid molecular technique for fungal species identification.
To analyze a specific fragment of mitochondrial DNA (mtDNA) for taxonomic differentiation.
To compare the efficiency of newly designed primer pairs with existing methods.

Summary:

A 1450 bp fragment of fungal mitochondrial DNA (mtDNA), encoding ND4, ATP6, and SsrDNA, along with adjacent SsrRNA 3' termini, was analyzed.
Three primer pairs were utilized, with one modified primer pair amplifying the SSrRNA gene region, as described by Li et al.
This molecular approach aims to identify differences at the fungal species level.

Impact:

Provides a potentially faster and more efficient method for fungal species identification.
Contributes to resolving taxonomic ambiguities in fungi using molecular data.
Offers a valuable tool for mycologists and researchers in fungal classification.