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Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
Expression of a Canavalia brasiliensis lectin (ConBr) precursor in Pichia pastoris
C P S Carvalho1, C S Rocha, D R Nepomuceno
1Laboratório de Genética Molecular, Departamento de Biologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
Protein and Peptide Letters
|May 14, 2008
Abstract:
A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.
