Related Experiment Video
Updated: Jul 5, 2026

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion
Published on: September 2, 2016
Production and recovery of polyunsaturated fatty acids-added lipids from fermented canola
1Biosystems Engineering, Clemson University, Biosystems Research Complex, 51 New Cherry Street, Clemson, SC 29634, USA.
Abstract:
Canola flake was investigated as a potential substrate for fungal conversion to produce polyunsaturated fatty acids (PUFAs)-added oil in a 7l fermenter. The results showed that yields of total oil were reduced 9-22% compared to initial oil in the canola flake, but as high as 445mg/l arachidonic acid (ARA, C20:4n6) and 67mg/l eicosapentaenoic acid (EPA, C20:5n3) were produced. The percentages of ARA and EPA of total fatty acids in this fermented oil were 15.5% and 2.3%, respectively. Supercritical CO2 extraction was then investigated for the lipid recovery from fermented canola flake, and extraction kinetics were modeled. The feasibility was demonstrated for production of PUFAs in a laboratory-scale fermentor using canola flake as a single nutrient, and for lipid extraction using supercritical CO2.
Related Concept Videos
Biofuels
Production of Organic Acids
Production of Alcohol
Structure of Lipids
Biosynthesis of Lipids
Microbial Bioremediation of Hydrocarbons

