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Related Experiment Video

Updated: Jul 5, 2026

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
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A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

Published on: September 9, 2015

BSAC standardized disc susceptibility testing method (version 7).

J M Andrews1,

  • 1Department of Microbiology, City Hospital NHS Trust, Birmingham B18 7QH, UK. jenny.andrews@swbh.nhs.uk

The Journal of Antimicrobial Chemotherapy
|May 14, 2008
PubMed
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This update revises antimicrobial susceptibility testing recommendations, including new breakpoints for various bacteria like Acinetobacter spp. and Stenotrophomonas maltophilia. It clarifies testing methods and antibiotic usage for improved clinical guidance.

Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Antimicrobial Resistance

Background:

  • Antimicrobial susceptibility testing (AST) is crucial for guiding effective antibiotic therapy.
  • Regular updates to AST guidelines are necessary to reflect evolving resistance patterns and new antimicrobial agents.
  • Version 6 of the recommendations provided a framework for AST, but required revisions based on new data and clinical insights.

Purpose of the Study:

  • To detail the specific changes and updates implemented in the latest version of antimicrobial susceptibility testing recommendations.
  • To provide clear guidance on revised testing conditions, breakpoints, and antibiotic interpretations for a range of clinically significant microorganisms.
  • To ensure clinicians and laboratorians have access to the most current and accurate information for accurate susceptibility testing.

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Published on: August 11, 2018

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Last Updated: Jul 5, 2026

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
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A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

Published on: September 9, 2015

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
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Main Methods:

  • The study involved a comprehensive review and revision of existing AST guidelines.
  • Specific changes include modifications to media and incubation conditions for Acinetobacter spp.
  • New or revised breakpoints (MIC and zone diameter) were established for various antibiotics and bacterial species, including cefoxitin, co-trimoxazole, daptomycin, and fluoroquinolones.

Main Results:

  • Updated recommendations cover testing for Acinetobacter spp., coagulase-negative staphylococci, Stenotrophomonas maltophilia, and Gram-negative rods from urinary tract infections.
  • Clarifications were provided regarding the use of specific antibiotics (e.g., cefoxitin, azithromycin, cefuroxime, erythromycin) and their interpretation in different clinical contexts.
  • Revised breakpoints and testing criteria were established for several antibiotics, including co-trimoxazole, daptomycin, neomycin, linezolid, ciprofloxacin, and vancomycin, with specific attention to Gram-negative rods and coliforms.

Conclusions:

  • The updated recommendations provide enhanced clarity and accuracy for antimicrobial susceptibility testing.
  • These revisions aim to improve the detection of antimicrobial resistance and support appropriate treatment decisions.
  • The continuous refinement of AST guidelines is essential for combating antimicrobial resistance and optimizing patient outcomes.