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Expression of novel genes encoded by the paramyxovirus J virus

Philippa J M Jack1, Danielle E Anderson1, Katharine N Bossart1

  • 1CSIRO Livestock Industries, Australian Animal Health Laboratory, Private Bag 24 Geelong, Victoria 3220, Australia.

The Journal of General Virology
|May 14, 2008
PubMed

Insights

This study confirms gene expression for the small hydrophobic (SH) and transmembrane (TM) proteins of J paramyxovirus (JPV). However, expression of the novel ORF-X was not detected, despite its presence in the JPV genome.

Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • J paramyxovirus (JPV) possesses a unique eight-gene genome structure.
  • The genome includes genes for small hydrophobic (SH), transmembrane (TM), and attachment (G) proteins, with a novel open reading frame (ORF-X) associated with the G gene.

Purpose of the Study:

  • To confirm the gene allocation within the JPV genome based on sequence data.
  • To determine the expression of proteins encoded by the SH, TM, and ORF-X genes.

Main Methods:

  • Northern blot analyses were used to examine mRNA transcripts from JPV-infected cells.
  • Specific probes were employed to detect mRNA species for the G protein and ORF-X.
  • Protein detection experiments were conducted to identify expressed viral proteins.

Main Results:

  • Transcription initiation and termination signals for SH and TM genes are functional, confirming their gene status, though read-through transcription occurs.
  • mRNA species corresponding to the predicted G protein gene length were detected, validating sequence predictions.
  • Both SH and TM proteins were detected in infected cells, but ORF-X expression was not observed.

Conclusions:

  • The study validates the gene allocation for SH and TM proteins in JPV.
  • The novel TM protein is identified as a type II glycosylated integral membrane protein.
  • Expression of the ORF-X remains unconfirmed, suggesting it may not be translated.

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