Related Experiment Video

Updated: Jul 5, 2026

Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
09:26

Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung

Published on: January 22, 2021

Accumulation of 99mTc-ciprofloxacin in Staphylococcus aureus and Pseudomonas aeruginosa

J M Sierra1, D Rodriguez-Puig, A Soriano

  • 1Department of Infectious Diseases, Hospital Clinic, IDIBAPS, School of Medicine, University of Barcelona, Barcelona, Spain. jmsierra@clinic.ub.es

Antimicrobial Agents and Chemotherapy
|May 14, 2008
PubMed

Insights

Technetium-99m-ciprofloxacin uptake was studied in bacteria causing infections. Overexpression of efflux pumps affected radiopharmaceutical accumulation, impacting diagnostic potential for bacterial infections.

Area of Science:

  • Nuclear medicine
  • Microbiology
  • Infectious diseases

Background:

  • Radiopharmaceuticals offer advanced methods for diagnosing infections by differentiating septic from aseptic inflammation.
  • Understanding radiopharmaceutical behavior in bacteria is crucial for accurate infection imaging.

Purpose of the Study:

  • To investigate the intracellular accumulation of technetium-99m-ciprofloxacin.
  • To analyze the impact of specific efflux pump overexpression on radiopharmaceutical uptake in bacterial strains.

Main Methods:

  • Utilized technetium-99m-ciprofloxacin as a radiopharmaceutical agent.
  • Examined Staphylococcus aureus strains overexpressing the NorA efflux pump.
  • Studied Pseudomonas aeruginosa strains overexpressing the MexAB-OprM efflux pump.

Main Results:

  • Observed varying intracellular accumulation of technetium-99m-ciprofloxacin based on bacterial strain and efflux pump status.
  • Efflux pump overexpression influenced the extent of radiopharmaceutical accumulation within bacterial cells.

Conclusions:

  • Bacterial efflux pump activity can significantly affect the intracellular accumulation of technetium-99m-ciprofloxacin.
  • These findings are important for optimizing radiopharmaceuticals used in diagnosing bacterial infections.

More Related Videos

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes08:23

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
Inhibitors of Bacterial DNA Synthesis01:28

Inhibitors of Bacterial DNA Synthesis

Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These antibiotics are selectively...