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Updated: Jul 5, 2026

Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats
Published on: December 5, 2017
Cyclic-AMP mediated drugs: differential or global reduction of eicosanoid synthesis in the isolated rat lung?
M J Post1, J D Biesebeek, J Wemer
1Laboratory for Experimental Cardiology Department of Cardiology University Hospital Utrecht HPN E 02.562 P.O. Box 85500 Utrecht 3508 GA The Netherlands.
Abstract:
In this study the question was addressed whether cAMP mediated drugs induce a differential reduction of branches of the arachidonic acid metabolism rather than a global reduction of eicosanoid synthesis. The isolated lungs of actively sensitized rats were employed to study prostaglandin and leukotriene release in the presence and absence of the cAMP mediated drugs theophylline, milrinone, sulmazole, isobutyl-methylxanthine and salbutamol. The release of eicosanoids as measured by RIA was predominantly basal and continuous, with a mild antigen induced stimulation only for TXB(2) and the leukotrienes. All drugs reduced eicosanoid release globally. It is concluded that cAMP mediated drugs interfere with arachidonic acid metabolism at a site proximal to the branching into lipoxygenase and cyclo-oxygenase pathways.
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