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Published on: September 24, 2011
Five steps in leukocyte extravasation in the microcirculation by chemoattractants
1Department of Pharmacology Kitasato University School of Medicine Kanagawa Sagamihara 228 Japan.
Mediators of Inflammation
|January 1, 1992
Summary
This study quantifies polymorphonuclear leukocyte (PMN) extravasation in vivo. Chemoattractants like leukotriene B4 promote PMN adhesion and migration through venular walls, detailing a five-step process.
Area of Science:
- Immunology
- Microcirculation Research
- Cell Biology
Background:
- In vitro studies suggest chemoattractants influence leukocyte behavior.
- Understanding in vivo leukocyte extravasation is crucial for inflammatory response research.
Purpose of the Study:
- To quantitatively analyze polymorphonuclear leukocyte (PMN) extravasation in vivo.
- To elucidate the sequential steps of PMN migration from the vascular lumen to the extravascular space.
Main Methods:
- Utilized a video system for quantitative analysis of PMN extravasation in the hamster cheek pouch microcirculation.
- Topically applied chemoattractants: leukotriene B4 and N-formyl-methionylleucyl-phenylalanine.
Main Results:
- Chemoattractants dose-dependently increased PMN adhesion to venules.
- 80-90% of adhered PMNs migrated into the venular wall within 10-12 minutes.
- PMNs remained in the venular wall for over 30 minutes before entering the extravascular space.
Conclusions:
- PMN extravasation is a dynamic, multi-step process.
- The process involves rolling, adhesion, transmigration through endothelium, retention in the venular wall, and basement membrane passage.
- This in vivo model provides a detailed framework for studying inflammatory cell migration.
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