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Murine Model of Intestinal Ischemia-reperfusion Injury
07:07

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Published on: May 11, 2016

Platelet activating factor synthesis and metabolism in intestinal ischaemia-reperfusion injury

M J Mangino1, M Murphy, A Bohrer

  • 1Departments of Surgery, Pathology and Medicine Washington University School of Medicine St. Louis Missouri 63110 USA.

Mediators of Inflammation
|January 1, 1994
PubMed

The object of this study was to characterize the synthesis and metabolism of platelet activating factor (PAF) by intestinal mucosa subjected to ischaemia-reperfusion injury. Canine intestinal mucosa produced 16:0-PAF, 18:0-PAF, and high levels of the corresponding lyso- PAF metabolites. Three h of intestinal ischaemia and ischaemia followed by 1 h of reperfusion did not affect the synthesis or metabolism of PAF by intestinal mucosa. Intestinal mucosa elaborated a factor that rapidly hydrolyzes PAF to lyso-PAF. The observed hydrolysis rate was not altered by ischaemia or ischaemia and reperfusion. In conclusion, this study suggests that intestinal mucosa produces PAF and rapidly hydrolyzes PAF. The PAF synthesis and metabolism rates of intestinal mucosa is not altered by ischaemia reperfusion in this model under the imposed conditions.

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