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Updated: Jul 5, 2026

Immunofluorescent Labeling in Nasal Mucosa Tissue Sections of Allergic Rhinitis Rats via Multicolor Immunoassay
Published on: September 22, 2023
Tumour necrosis factor-alpha in nasal allergy
K Hisamatsu1, T Ganbo, T Nakazawa
1Department of Otorhinolaryngology Yamanashi Medical University 1110 Shimokato Tamaho-cho, Nakakoma-gun Yamanashi-ken 409-38 Japan.
Abstract:
Tumour necrosis factor-alpha (TNF-alpha) was measured by enzyme-linked immunosorbent assay and eosinophil cationic protein (ECP) by radio-immunoassay to evaluate TNF-alpha in nasal allergy. There was no significant difference either between the mean concentrations of TNF-alpha in nasal secretions from the patients with perennial nasal allergy and those of normal subjects, or between the TNF-alpha and ECP concentrations. However, reverse transcription polymerase chain reaction showed a specific increase of TNF-alpha mRNA and IFN-gamma mRNA in allergic nasal mucosa after allergen challenge in vitro. These findings suggest a possibility that T cell-derived IFN-gamma up-regulates macrophages to elaborate TNF-alpha, which may play a role in amplifying allergic inflammation in the nose through the cytokine network.
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