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Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells

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Published on: December 18, 2010

Granulocyte colony-stimulating factor in amniotic fluid.

B D Raynor¹, P Clark, P Duff

¹Division of Maternal Fetal Medicine Department of Obstetrics and Gynecology University of Florida, Gainesville, FL, USA.

Infectious Diseases in Obstetrics and Gynecology

|January 1, 1995

Summary

Granulocyte colony-stimulating factor (G-CSF) is present in amniotic fluid and increases with labor. Intrauterine infection (chorioamnionitis) significantly elevates G-CSF levels during labor.

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Area of Science:

Reproductive Biology
Immunology
Obstetrics

Background:

Granulocyte colony-stimulating factor (G-CSF) plays a crucial role in immune responses.
The presence and regulation of G-CSF in amniotic fluid during pregnancy and labor are not fully understood.

Purpose of the Study:

To investigate the normal presence of G-CSF in amniotic fluid.
To determine the impact of labor and intrauterine infection on amniotic fluid G-CSF levels.

Main Methods:

Amniotic fluid samples were collected from 35 patients categorized into four groups: no labor, early labor, late labor, and labor with chorioamnionitis.
G-CSF concentrations were quantified using enzyme-linked immunosorbent assay (ELISA).

Main Results:

Mean amniotic fluid G-CSF levels were significantly lower before labor compared to during labor (0.49 vs. 1.83 ng/ml, P < 0.001).
G-CSF levels increased progressively from no labor to early and late labor.
In cases of chorioamnionitis, G-CSF levels were markedly elevated compared to normal labor (25.0 vs. 1.83 ng/ml, P < 0.0001).

Conclusions:

G-CSF is a normal component of amniotic fluid.
Labor is associated with increased amniotic fluid G-CSF concentrations.
Intrauterine infection (chorioamnionitis) leads to a significant elevation of G-CSF in amniotic fluid during labor.