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Updated: Jul 5, 2026
Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection
Published on: August 11, 2023
[Amplification methods of DNA from paraffin-embedded tissues of oral leukoplakia]
Yuan He1, Qian-ming Chen, Bing-qi Li
1Department of Oral Medicine, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Objective:
To set up a feasible method for detection of objective genes from paraffin-embedded tissues of oral leukoplakia (OLK).
Methods:
Twenty-five pieces of formalin-fixed, paraffin-embedded tissues of OSCC were selected and ATM gene was detected respectively by 3 methods: the microdissection-nested PCR method, proteinase K-PCR method and conventional phenol-chloroform-PCR method. The positivity rates were compared statistically.
Results:
The positivity rates of these 3 methods were 84%, 52% and 64% respectively. Significant difference was found in positivity rate between the microdissection-nested PCR method and the proteinase K-PCR method (P = 0.032).
Conclusions:
The microdissection-nested PCR method merits recommendation because it is more efficient, easy to perform and has the advantage of less sample amount.
