Quantitative surface plasmon resonance imaging: a simple approach to automated angle scanning

Area of Science:

• Surface science
• Optical instrumentation
• Analytical chemistry

Background:

• Surface plasmon resonance imaging (SPRi) is a powerful technique for analyzing surface interactions.
• Existing fixed-angle SPRi instruments have limitations in dynamic range and versatility.
• Angle-dependent artifacts can complicate data interpretation in SPRi.

Purpose of the Study:

• To develop an automated angle-scanning SPRi instrument for multiplexed, quantitative reflectance measurements.
• To overcome the dynamic range limitations of fixed-angle SPRi instruments.
• To enhance the flexibility and sensitivity of SPRi for studying diverse surface systems.

Main Methods:

• An automated angle-scanning SPRi instrument was designed and implemented.
• Software-based correction of angle-dependent artifacts was developed.
• Data analysis involved fitting reflectance data to the Fresnel model.
• Operation across the visible to near-infrared spectrum was achieved without reconfiguration.

Main Results:

• The instrument provides multiplexed, quantitative reflectance data over a wide angular range.
• Software correction effectively addressed angle-dependent artifacts.
• The system enables monitoring of surface coatings in various solvents, exceeding the dynamic range of fixed-angle instruments.
• Extended spectral range enhances sensitivity and allows investigation of surface film optical properties.

Conclusions:

• The automated angle-scanning SPRi instrument offers enhanced flexibility and quantitative capabilities.
• This technology facilitates the study of a wide range of surface systems and coatings.
• The instrument's design overcomes limitations of traditional SPRi, enabling broader applications in surface science and materials analysis.