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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.

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Related Experiment Video

Updated: Jul 5, 2026

Monochrome Multiplex Quantitative PCR Telomere Length Measurement
11:44

Monochrome Multiplex Quantitative PCR Telomere Length Measurement

Published on: March 22, 2024

A quantitative real-time PCR method for absolute telomere length.

Nathan O'Callaghan1, Varinderpal Dhillon, Philip Thomas

  • 1Commonwealth Scientific and Industrial Research Organization (CSIRO)-Human Nutrition, Adelaide, Australia. nathan.o'callaghan@csiro.au

Biotechniques
|May 15, 2008
PubMed
Summary
This summary is machine-generated.

We developed a new method to accurately measure absolute telomere length, a key factor in aging and cancer. This technique provides reliable, comparable results for researchers studying telomere dynamics.

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Last Updated: Jul 5, 2026

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Optimization of Performance Parameters of the TAGGG Telomere Length Assay
08:23

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Telomere shortening is a significant risk factor associated with cancer development and accelerated aging.
  • Accurate measurement of telomere length is crucial for understanding these processes.
  • Existing methods may lack reproducibility or provide only relative quantification.

Purpose of the Study:

  • To develop a simple, reproducible method for measuring absolute telomere length.
  • To improve upon existing quantitative real-time PCR (qRT-PCR) assays for telomere measurement.
  • To enable more direct comparison of telomere length data across studies.

Main Methods:

  • Adaptation of Cawthon's quantitative real-time PCR (qRT-PCR) assay.
  • Incorporation of an oligomer standard for absolute quantification.
  • Validation against the gold standard terminal restriction fragment (TRF) analysis via Southern hybridization.

Main Results:

  • The developed method accurately measures absolute telomere length.
  • A strong correlation was observed between the new method and TRF analysis.
  • The method provides absolute telomere length values, not just relative quantification.

Conclusions:

  • The new method offers a simple and reproducible way to determine absolute telomere length.
  • This advancement facilitates more consistent and comparable research findings in telomere biology.
  • Improved telomere length measurement can advance research in aging and cancer.