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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scaleĀ  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Globular and Fibrous Proteins02:21

Globular and Fibrous Proteins

Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...

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Related Experiment Video

Updated: Jul 5, 2026

Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery
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Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery

Published on: October 4, 2024

Silkworm nucleotide databases--current trends and future prospects.

Nicole Koshy1, Kangayam M Ponnuvel, Randhir K Sinha

  • 1Biotechnology Laboratory, Central Sericulture and Germplasm research Centre, P.O. Box: 34, Thally road, Hosur-635109, Tamil Nadu, India.

Bioinformation
|May 15, 2008
PubMed
Summary
This summary is machine-generated.

The silkworm (Bombyx mori) genome databases are valuable for lepidopteran research, but protein data is scarce. Future efforts should focus on expanding these resources for broader insect biological studies.

Keywords:
Bombyx morilepidopteranssilk knowledgebasesilkbasesilkworm

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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

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Last Updated: Jul 5, 2026

Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery
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Larval RNA Interference in Silkworm Bombyx mori through Chitosan/dsRNA Nanoparticle Delivery

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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

Area of Science:

  • * Entomology
  • * Genomics
  • * Bioinformatics

Background:

  • * The domesticated silkworm, Bombyx mori, is a key model organism in lepidopteran research.
  • * Several databases exist for silkworm genomic data, including nucleotide sequences, expressed sequence tags (ESTs), and microsatellites.
  • * These databases also incorporate data for other lepidopteran and insect species, supporting broader entomological research.

Purpose of the Study:

  • * To review the current status of nucleotide databases for the silkworm (Bombyx mori).
  • * To identify limitations and suggest improvements for existing silkworm and lepidopteran databases.
  • * To explore future possibilities for developing new databases, particularly for protein information.

Main Methods:

  • * Comprehensive review of existing silkworm and lepidopteran nucleotide databases.
  • * Analysis of data content, accessibility, and update frequency.
  • * Identification of data gaps, specifically in protein-related information.

Main Results:

  • * Four primary databases are crucial for storing silkworm nucleotide information (genomic data, ESTs, microsatellites).
  • * While nucleotide data is abundant, there is a significant lack of protein data for silkworms and other lepidopterans.
  • * Existing databases facilitate insect biological research by including data from related species.

Conclusions:

  • * Current nucleotide databases are essential but require expansion, especially for protein data.
  • * Future database development should prioritize filling the protein data gap for silkworms and lepidopterans.
  • * Enhanced databases will significantly advance insect biological and genomic research.